Analytical Data
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基因名
hopM1
- Application
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别名
Hrp outer protein M1 (Type III effector HopPtoM) (holPtoX) (hopPtoM)
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种属
Pseudomonas syringae pv. tomato
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表达系统
E. coli
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标签
N- His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q887D0
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表达区间
1-712aa
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分子量
81.3 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
HopM1 is a key effector protein from the plant pathogen *Pseudomonas syringae*, playing a critical role in the bacterium's ability to induce disease in plants such as Arabidopsis thaliana. This protein is part of a broader class of type III secretion system (T3SS) effectors that facilitate the manipulation of host cellular processes to promote pathogen survival and reproduction. Specifically, HopM1 has been implicated in disrupting normal plant immune responses, enabling bacterial colonization. Research has highlighted its ability to interact with host proteins, including those involved in the plant's signaling pathways, which suggests a sophisticated mechanism of pathogenesis. Studies on the HopM1 protein can provide valuable insights into plant-pathogen interactions and the molecular basis of disease resistance, with implications for developing strategies to enhance plant resilience against bacterial infections. Understanding the structural and functional properties of HopM1 could lead to novel approaches in agricultural biotechnology, aimed at engineering disease-resistant crop varieties. As such, the study of HopM1 represents a pivotal area within plant biology and microbiology, bridging the gap between fundamental research and practical applications in crop protection and management.
