Analytical Data
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基因名
BCAT1
- Application
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别名
BCAT1；BCT1；ECA39；；Branched-chain-amino-acid aminotransferase. cytosolic
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种属
Human
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表达系统
E. coli
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标签
Sumo+N-6His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P54687
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表达区间
1-386aa
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氨基酸序列
MGSSHHHHHH SSGLVPRGSH MGSMKDCSNG CSAECTGEGG SKEVVGTFKA KDLIVTPATI LKEKPDPNNL VFGTVFTDHM LTVEWSSEFG WEKPHIKPLQ NLSLHPGSSA LHYAVELFEG LKAFRGVDNK IRLFQPNLNM DRMYRSAVRA TLPVFDKEEL LECIQQLVKL DQEWVPYSTS ASLYIRPTFI GTEPSLGVKK PTKALLFVLL SPVGPYFSSG TFNPVSLWAN PKYVRAWKGG TGDCKMGGNY GSSLFAQCEA VDNGCQQVLW LYGEDHQITE VGTMNLFLYW INEDGEEELA TPPLDGIILP GVTRRCILDL AHQWGEFKVS ERYLTMDDLT TALEGNRVRE MFGSGTACVV CPVSDILYKG ETIHIPTMEN GPKLASRILS KLTDIQYGRE ESDWTIVLS
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蛋白长度
Full Length of Mature Protein
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分子量
60 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
BCAT1 (branched-chain amino acid transaminase 1) is an enzyme that plays a pivotal role in the metabolism of branched-chain amino acids (BCAAs), specifically leucine, isoleucine, and valine. This enzyme is primarily located in the mitochondria and is crucial for maintaining amino acid homeostasis, which is essential for various physiological processes, including protein synthesis, energy production, and metabolic regulation. Alterations in BCAT1 activity have been implicated in several pathophysiological conditions, including cancer, where its upregulation is often associated with tumor growth and progression. The study of BCAT1 recombination protein has garnered attention due to its potential as a therapeutic target. By harnessing recombinant techniques, researchers aim to produce purified BCAT1 proteins for a variety of applications, including structural biology, enzyme kinetics, and the development of inhibitors. Understanding the structure-function relationship of BCAT1 and its interactions with other biomolecules can shed light on its role in disease mechanisms and metabolic disorders. Moreover, investigating its catalytic mechanism may facilitate the discovery of novel drugs that can modulate BCAA metabolism, offering new avenues for treatment strategies in diseases where BCAA dysregulation is a contributing factor. Thus, the exploration of BCAT1 recombinant proteins is not only pivotal for expanding our knowledge of amino acid metabolism but also holds potential implications for therapeutic advancements in cancer and other metabolic diseases.
