Analytical Data
-
Gene name
BCAT1
- Application
-
Alternative Names
BCAT1；BCT1；ECA39；；Branched-chain-amino-acid aminotransferase. cytosolic
-
Species
Human
-
Source
E. coli
-
Tag
Sumo+N-6His
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
P54687
-
Expression Region
1-386aa
-
AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSMKDCSNG CSAECTGEGG SKEVVGTFKA KDLIVTPATI LKEKPDPNNL VFGTVFTDHM LTVEWSSEFG WEKPHIKPLQ NLSLHPGSSA LHYAVELFEG LKAFRGVDNK IRLFQPNLNM DRMYRSAVRA TLPVFDKEEL LECIQQLVKL DQEWVPYSTS ASLYIRPTFI GTEPSLGVKK PTKALLFVLL SPVGPYFSSG TFNPVSLWAN PKYVRAWKGG TGDCKMGGNY GSSLFAQCEA VDNGCQQVLW LYGEDHQITE VGTMNLFLYW INEDGEEELA TPPLDGIILP GVTRRCILDL AHQWGEFKVS ERYLTMDDLT TALEGNRVRE MFGSGTACVV CPVSDILYKG ETIHIPTMEN GPKLASRILS KLTDIQYGRE ESDWTIVLS
-
Protein Length
Full Length of Mature Protein
-
Molecular Weight
60 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
BCAT1 (branched-chain amino acid transaminase 1) is an enzyme that plays a pivotal role in the metabolism of branched-chain amino acids (BCAAs), specifically leucine, isoleucine, and valine. This enzyme is primarily located in the mitochondria and is crucial for maintaining amino acid homeostasis, which is essential for various physiological processes, including protein synthesis, energy production, and metabolic regulation. Alterations in BCAT1 activity have been implicated in several pathophysiological conditions, including cancer, where its upregulation is often associated with tumor growth and progression. The study of BCAT1 recombination protein has garnered attention due to its potential as a therapeutic target. By harnessing recombinant techniques, researchers aim to produce purified BCAT1 proteins for a variety of applications, including structural biology, enzyme kinetics, and the development of inhibitors. Understanding the structure-function relationship of BCAT1 and its interactions with other biomolecules can shed light on its role in disease mechanisms and metabolic disorders. Moreover, investigating its catalytic mechanism may facilitate the discovery of novel drugs that can modulate BCAA metabolism, offering new avenues for treatment strategies in diseases where BCAA dysregulation is a contributing factor. Thus, the exploration of BCAT1 recombinant proteins is not only pivotal for expanding our knowledge of amino acid metabolism but also holds potential implications for therapeutic advancements in cancer and other metabolic diseases.
