Analytical Data
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Gene name
icaB
- Application
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Alternative Names
Biofilm polysaccharide intercellular adhesin deacetylase Short name: Biofilm PIA deacetylase Intercellular adhesion protein B
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Species
Staphylococcus aureus
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Source
E. coli
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Tag
N- His & C- Myc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q7A349
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Expression Region
29-290aa
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Molecular Weight
35.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The study of the icaB gene and its recombinant protein has gained significant attention in the field of microbiology and infectious disease research, particularly concerning Staphylococcus aureus, a major human pathogen. The icaAB operon is crucial for the biosynthesis of polysaccharide intercellular adhesin (PIA), a key component in biofilm formation that enhances the survival and virulence of bacterial infections, especially in hospital settings. As antibiotic resistance continues to rise, understanding the mechanisms of biofilm development and stability is imperative for developing effective treatments. Recombinant IcaB protein serves as a valuable tool for elucidating the functional characteristics of the Ica system and its role in biofilm formation. By producing and characterizing IcaB, researchers aim to uncover potential targets for disrupting biofilm integrity, thereby enhancing the efficacy of existing antibiotics and developing novel therapeutic strategies. Additionally, investigations into the immunogenic properties of recombinant IcaB may offer insights into vaccine development against S. aureus infections. Overall, the continued exploration of icaB and its recombinant protein is crucial for advancing our understanding of microbial pathogenesis and improving outcomes in treating bacterial infections.
