Analytical Data
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基因名
ATP5D
- Application
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别名
ATP5D;ATP5D;ATP synthase subunit delta. mitochondrial
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P30049
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表达区间
23-168aa
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氨基酸序列
MGSSHHHHHHSSGLVPRGSHMAEAAAAPAAASGPNQMSFTFASPTQVFFN GANVRQVDVPTLTGAFGILAAHVPTLQVLRPGLVVVHAEDGTTSKYFVSS GSIAVNADSSVQLLAEEAVTLDMLDLGAAKANLEKAQAELVGTADEATRA EIQIRIEANEALVKALE
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分子量
17 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ATP5D, a subunit of the ATP synthase complex, plays a crucial role in cellular energy production through oxidative phosphorylation. This protein is part of the F1 sector of the ATP synthase, which synthesizes adenosine triphosphate (ATP) using the proton gradient generated across the inner mitochondrial membrane. Research has indicated that ATP5D not only contributes to ATP synthesis but also has implications in various cellular processes, including regulation of apoptosis and cell proliferation. Mutations or dysregulation of ATP5D have been linked to several diseases, particularly mitochondrial disorders and certain types of cancer, highlighting its significance in maintaining cellular energy homeostasis and overall mitochondrial function. Recombinant ATP5D protein has become a focal point for experimental studies, enabling researchers to elucidate its structural and functional properties. The production and characterization of ATP5D in recombinant systems have facilitated a deeper understanding of its role in mitochondrial bioenergetics and its potential as a therapeutic target. Additionally, the investigation of ATP5D interactions with other subunits of ATP synthase and its impact on enzyme activity and assembly provides insights into the mechanisms of ATP production and mitochondrial dynamics. As research progresses, the exploration of ATP5D as a biomarker for disease and a target for drug development continues to expand, underscoring its relevance in both basic and clinical research settings.












