Analytical Data
-
基因名
AGK
- Application
-
别名
AGK；MULK；Acylglycerol kinase. mitochondrial
-
种属
Human
-
表达系统
E. coli
-
标签
His tag N-Terminus
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
Q53H12
-
表达区间
1-422aa
-
氨基酸序列
MTVFFKTLRNHWKKTTAGLCLLTWGGHWLYGKHCDNLLRRAACQEAQVFGNQLIPPNAQVKKATVFLNPAACKGKARTLFEKNAAPILHLSGMDVTIVKTDYEGQAKKLLELMENTDVIIVAGGDGTLQEVVTGVLRRTDEATFSKIPIGFIPLGETSSLSHTLFAESGNKVQHITDATLAIVKGETVPLDVLQIKGEKEQPVFAMTGLRWGSFRDAGVKVSKYWYLGPLKIKAAHFFSTLKEWPQTHQASISYTGPTERPPNEPEETPVQRPSLYRRILRRLASYWAQPQDALSQEVSPEVWKDVQLSTIELSITTRNNQLDPTSKEDFLNICIEPDTISKGDFITIGSRKVRNPKLHVEGTECLQASQCTLLIPEGAGGSFSIDSEEYEAMPVEVKLLPRKLQFFCDPRKREQMLTSPTQ
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
AGK (Acylglycerol Kinase) is a crucial enzyme that plays a pivotal role in lipid metabolism by catalyzing the phosphorylation of monoacylglycerol to produce diacylglycerol, an important lipid signaling molecule. The study of recombinant AGK proteins has gained significant attention due to their potential implications in understanding various metabolic disorders, including obesity and diabetes. The ability to produce AGK in a recombinant form allows researchers to investigate its structural and functional properties more effectively, facilitating insights into its enzymatic mechanisms and regulation. Moreover, recombinant AGK proteins serve as valuable tools in drug discovery, particularly in the development of inhibitors that can modulate AGK activity for therapeutic interventions. As lipid metabolism is intricately linked to cellular signaling pathways, studying AGK can also contribute to our understanding of broader biological processes, including inflammation and cancer. Research is ongoing to explore the optimal conditions for AGK expression, purification, and characterization, aiming to elucidate its role in lipid signaling and its potential as a target for therapeutic applications.
