Analytical Data
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基因名
ATG5
- Application
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别名
ATG5；APG5L；ASP；Autophagy Protein 5
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9H1Y0
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表达区间
1-275aa
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氨基酸序列
MGSSHHHHHHSSGLVPRGSHMTDDKDVLRDVWFGRIPTCFTLYQDEITER EAEPYYLLLPRVSYLTLVTDKVKKHFQKVMRQEDISEIWFEYEGTPLKWH YPIGLLFDLLASSSALPWNITVHFKSFPEKDLLHCPSKDAIEAHFMSCMK EADALKHKSQVINEMQKKDHKQLWMGLQNDRFDQFWAINRKLMEYPAEEN GFRYIPFRIYQTTTERPFIQKLFRPVAADGQLHTLGDLLKEVCPSAIDPE DGEKKNQVMIHGIEPMLETPLQWLSEHLSYPDNFLHISIIPQPTD
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分子量
35 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ATG5, a vital autophagy-related protein, plays a crucial role in the cellular process of autophagy, which is essential for maintaining cellular homeostasis, degrading damaged organelles, and responding to stress. Autophagy is implicated in various physiological processes, including metabolism, immunity, and development, and is linked to the pathogenesis of numerous diseases such as neurodegeneration, cancer, and infections. ATG5 functions mainly in the formation of autophagosomes, and its activity is regulated by several signaling pathways. Research on recombinant ATG5 protein focuses on understanding the mechanistic details of autophagy and its regulation. By producing and studying recombinant ATG5, researchers aim to elucidate its interaction with other autophagy-related proteins and the downstream effects on cellular function. Furthermore, the investigation of ATG5's role in disease models may provide insights into therapeutic strategies, as modulation of autophagy represents a promising avenue for treating conditions associated with dysregulated autophagic processes. Given the intricate relationship between ATG5 and health, the study of this recombinant protein has gained attention in the fields of cell biology and biomedical research, highlighting its significant potential in developing innovative approaches for tackling various diseases.
