Analytical Data
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Gene name
MTHFD1
- Application
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Alternative Names
Short name:C1-THF synthase
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Species
Pongo abelii
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Source
E. coli
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Tag
N- His-SUMO
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q5R8P0
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Expression Region
1-305aa
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Molecular Weight
49 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
MTHFD1 (Methylenetetrahydrofolate dehydrogenase 1) is an essential enzyme involved in folate metabolism, playing a critical role in cellular one-carbon metabolism, which is vital for nucleotide synthesis and cellular proliferation. This enzyme participates in the conversion of methylenetetrahydrofolate to methyltetrahydrofolate, linking them to various metabolic pathways that support cell growth and division. Abnormalities in MTHFD1 activity have been linked to several diseases, including cancer and developmental disorders, highlighting its importance in health and disease. Recent studies have focused on the recombinant expression of MTHFD1 to elucidate its structural and functional properties, aiming to understand its mechanism and interactions within metabolic networks. Recombinant MTHFD1 has been produced in various systems, enabling the investigation of its kinetic properties and the effects of specific inhibitors. Understanding MTHFD1 at a molecular level could provide insights into potential therapeutic targets for diseases associated with folate metabolism. Researchers are keen on manipulating this enzyme for applications in synthetic biology and bioengineering, further emphasizing the relevance of MTHFD1 in both fundamental research and clinical applications.
