Analytical Data
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基因名
ATG3
- Application
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别名
ATG3；APG3；APG3L；；Ubiquitin-like-conjugating enzyme ATG3
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9NT62
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表达区间
1-314aa
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氨基酸序列
MQNVINTVKGKALEVAEYLTPVLKESKFKETGVITPEEFVAAGDHLVHHC PTWQWATGEELKVKAYLPTGKQFLVTKNVPCYKRCKQMEYSDELEAIIEE DDGDGGWVDTYHNTGITGITEAVKEITLENKDNIRLQDCSALCEEEEDED EGEAADMEEYEESGLLETDEATLDTRKIVEACKAKTDAGGEDAILQTRTY DLYITYDKYYQTPRLWLFGYDEQRQPLTVEHMYEDISQDHVKKTVTIENH PHLPPPPMCSVHPCRHAEVMKKIIETVAEGGGELGVHMYL LIFLKFVQAVIPTIEYDYTR HFTM
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分子量
33 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ATG3, a crucial component of the autophagy machinery, plays a significant role in the formation of autophagosomes, which are vital for cellular degradation and recycling processes. Autophagy is an essential cellular process that helps maintain homeostasis by removing damaged organelles and misfolded proteins, thereby preventing the development of various diseases, including neurodegenerative disorders, cancer, and infections. Research on ATG3 has gained momentum due to its involvement in the ubiquitin-like conjugation systems that facilitate the lipidation of LC3, a core protein in autophagosome formation. Understanding the functional dynamics of ATG3 offers insights into the mechanisms underlying autophagy regulation, cellular stress responses, and the potential development of therapeutic strategies targeting autophagy-dependent pathways. Given that dysregulation of autophagy is associated with several diseases, the study of ATG3 and its interactions with other autophagy-related proteins is pivotal in deciphering its role in health and disease. Current studies are exploring the structural characteristics of ATG3, its regulatory mechanisms, and its potential as a target for drug development in diseases where autophagy is altered. This area of research not only enhances our understanding of fundamental cellular processes but also holds promise for innovative therapeutic approaches.
