Analytical Data
-
Gene name
ATG10
- Application
-
Alternative Names
ATG10；APG10L；Ubiquitin-like-conjugating enzyme ATG10
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
Q9H0Y0
-
Expression Region
1-220aa
-
AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSMEEDEFI GEKTFQRYCA EFIKHSQQIG DSWEWRPSKD CSDGYMCKIH FQIKNGSVMS HLGASTHGQT CLPMEEAFEL PLDDCEVIET AAASEVIKYE YHVLYSCSYQ VPVLYFRASF LDGRPLTLKD IWEGVHECYK MRLLQGPWDT ITQQEHPILG QPFFVLHPCK TNEFMTPVLK NSQKINKNVN YITSWLSIVG PVVGLNLPLS YAKATSQDER NVP
-
Molecular Weight
28 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ATG10 is an essential protein involved in the autophagy process, a cellular mechanism that degrades and recycles cytoplasmic components. Autophagy plays a critical role in maintaining cellular homeostasis, responding to stress, and regulating various physiological processes, including metabolism, immunity, and aging. The ATG10 protein specifically functions as an E2-like ubiquitin-conjugating enzyme, facilitating the conjugation of ubiquitin-like proteins to target substrates, thereby promoting the formation of autophagosomes. Dysregulation of autophagy has been implicated in numerous diseases, including cancer, neurodegenerative disorders, and infections. Understanding the role of ATG10 in autophagy is crucial for identifying potential therapeutic targets and developing novel treatments. Recent studies have revealed that ATG10 is also involved in the regulation of selective autophagy, which clears damaged organelles and misfolded proteins, further underscoring its significance in cellular health. Given these insights, researchers are increasingly focusing on the biochemical properties, modulatory mechanisms, and interaction networks of ATG10 to elucidate its contributions to autophagic processes and its potential implications in various diseases. This research aims to provide deeper insights into the molecular mechanisms governing autophagy and explore the possibility of manipulating ATG10 activity for therapeutic benefit.
