Analytical Data
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基因名
ATF4
- Application
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别名
ATF4；CREB2；TXREB；Cyclic AMP-dependent transcription factor ATF-4
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P18848
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表达区间
1-351aa
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氨基酸序列
MHHHHHHMADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQN PTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVF DKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFV QMMTAKGSHMTEMSFLSSEVLVGDLMSPFDQSGLGAEESLGLLDDYLEVA KHFKPHGFSSDKAKAGSSEWLAVDGLVSPSNNSKEDAFSGTDWMLEKMDL KEFDLDALLGIDDLETMPDDLLTTLDDTCDLFAPLVQETNKQPPQTVNPI GHLPESLTKPDQVAPFTFLQPLPLSPGVLSSTPDHSFSLELGSEVDITEG DRKPDYTAYVAMIPQCIKEEDTPSDNDSGICMSPESYLGSPQHSPSTRGS PNRSLPSPGVLCGSARPKPYDPPGEKMVAAKVKGEKLDKKLKKMEQNKTA ATRYRQKKRAEQEALTGECKELEKKNEALKERADSLAKEIQYLKDLIEEV RKARGKKRVP
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分子量
57 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ATF4 (Activating Transcription Factor 4) is a crucial transcription factor that plays a significant role in the cellular response to various stress conditions, including amino acid deprivation, oxidative stress, and endoplasmic reticulum (ER) stress. It is encoded by the ATF4 gene and is part of the ATF/CREB family of proteins. Under stress conditions, ATF4 is induced through the integrated stress response (ISR), primarily via phosphorylation of eIF2α, leading to changes in gene expression that promote adaptation, survival, and apoptosis in cells. Research has shown that ATF4 is not only pivotal in the stress response but also involved in metabolic regulation and differentiation processes. Notably, it has implications in various diseases, including cancer, neurodegenerative disorders, and metabolic syndromes, highlighting its importance in both basic and translational research. Scientists have increasingly focused on ATF4 reconstitution studies to better understand its functional mechanisms and regulatory roles. Recent advances in molecular techniques have facilitated the study of ATF4 protein, its post-translational modifications, and its interactions with other proteins within the cellular milieu. These studies aim to unravel the complexities of its function, with the potential for therapeutic interventions targeting ATF4 signaling pathways. Understanding ATF4's role in health and disease can provide valuable insights into novel strategies for disease management and therapeutic development.
