Analytical Data
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Gene name
CLC
- Application
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Alternative Names
CLC；Chloride channel Protein 2
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Species
E.coli
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9VWA1
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Expression Region
1-219aa
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AA Sequence
MDFGDDFAAKEDVDPAAEFLAREQSALGDLEAEITGGSASAPPAASTDEGLGELLGGTASEGDLLSAGGTGGLESSTGSFEVIGGESNEPVGISGPPPSREEPEKIRKWREEQKQRLEEKDIEEERKKEELRQQSKKELDDWLRQIGESISKTKLASRNAEKQAATLENGTIEPGTEWERIAKLCDFNPKVNKAGKDVSRMRSIYLHLKQNPIQVQKST
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Molecular Weight
23.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The study of CLC (chloride channels) recombinant proteins has gained significant attention in recent decades due to their vital roles in various physiological processes and their implications in various diseases. CLC proteins are part of a family of ion channels that primarily facilitate the transport of chloride ions across cellular membranes, which is crucial for maintaining cellular homeostasis, regulating cell volume, and conducting electrical signals in excitable tissues. Research has shown that dysfunctions in CLC channels are linked to a variety of disorders, including genetic diseases like myotonia congenital and cystic fibrosis, as well as conditions such as hypertension and certain types of cancer. The ability to produce recombinant CLC proteins has revolutionized the field, enabling scientists to investigate their structure-function relationships and the mechanisms underlying ion transport. Techniques such as molecular cloning, expression in heterologous systems, and electrophysiological assays have been employed to characterize these proteins in detail. By enhancing our understanding of CLC channels through recombinant technology, researchers are working to develop targeted therapies that address the underlying causes of CLC-related diseases, highlighting the importance of this field in both basic and applied biomedical research.
