Analytical Data
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Gene name
ERK1
- Application
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Alternative Names
ERK1；ERK1；PRKM3；Mitogen-activated Protein kinase 3
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Species
Human
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Source
E. coli
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Tag
N-6His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P27361
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Expression Region
2-379aa
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AA Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSAAAAAQGGGGGEPRRTEGV GPGVPGEVEMVKGQPFDVGPRYTQLQYIGEGAYGMVSSAYDHVRKTRVAI KKISPFEHQTYCQRTLREIQILLRFRHENVIGIRDILRASTLEAMRDVYI VQDLMETDLYKLLKSQQLSNDHICYFLYQILRGLKYIHSANVLHRDLKPS NLLSNTTCDLKICDFGLARIADPEHDHTGFLTEYVATRWYRAPEIMLNSK GYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLN CIINMKARNYLQSLPSKTKVAWAKLFPKSDSKALDLLDRMLTFNPNKRIT VEEALAHPYLEQYYDPTDEPVAEEPFTFAMELDDLPKERLKELIFQETAR FQPGVLEAP
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Molecular Weight
46 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Identification
Protein Description
The research on recombinant ERK1 protein (Extracellular Signal-Regulated Kinase 1) stems from its critical role in cellular signaling pathways, particularly the MAPK/ERK pathway, which is essential for various cellular processes including proliferation, differentiation, and survival. Abnormal regulation of ERK1 is implicated in numerous diseases, including cancer, where its persistent activation drives uncontrolled cell growth and tumor progression. Given its importance, there has been considerable interest in understanding the structure-function relationship of ERK1 and its interactions with other proteins within the signaling cascade. Recombinant technology has enabled the production of ERK1 in a controlled laboratory setting, facilitating detailed biochemical studies, functional assays, and potential therapeutic applications. These studies not only enhance our comprehension of ERK1's role in normal physiology but also pave the way for developing targeted therapies to modulate its activity in pathological conditions. The availability of purified recombinant ERK1 allows researchers to explore its substrate specificity, phosphorylation dynamics, and its influence on downstream effectors, thereby providing crucial insights into the molecular mechanisms underpinning various diseases and identifying novel intervention points for drug development.
