Analytical Data
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Gene name
RHOT1
- Application
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Alternative Names
MIRO-1 Alternative name(s): Ras homolog gene family member T1
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Species
Chicken
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Source
E. coli
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Tag
N- His-SUMO
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q5ZM73
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Expression Region
1-219aa
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Molecular Weight
41 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
RHOT1, a member of the Ras superfamily of small GTPases, has garnered increasing attention due to its crucial role in cellular processes such as mitochondrial dynamics, cell migration, and proliferation. Initially identified for its function in regulating organelle movement within cells, RHOT1 has been implicated in various pathological conditions, including cancer and neurodegenerative diseases. Research has demonstrated that RHOT1 interacts with multiple proteins involved in mitochondrial transport and fusion, highlighting its significance in maintaining mitochondrial integrity and function. Additionally, its dysregulation has been associated with altered cellular metabolism and enhanced tumorigenic properties. Given these implications, there is a growing interest in developing recombinant RHOT1 protein for further characterization studies that aim to elucidate its biological functions and regulatory mechanisms. Understanding RHOT1 at a molecular level may unveil new therapeutic targets and strategies for diseases linked to mitochondrial dysfunction and aberrant cell signaling pathways. Therefore, the production and study of RHOT1 recombinant protein is not only fundamental for basic scientific research but also for advancing our knowledge in clinical applications related to cancer and other disorders involving mitochondrial dynamics.
