Analytical Data
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Gene name
AS3MT
- Application
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Alternative Names
AS3MT；CYT19；Arsenite methyltransferase
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9HBK9
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Expression Region
1-375aa
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AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMAALRD AEIQKDVQTY YGQVLKRSAD LQTNGCVTTA RPVPKHIREA LQNVHEEVAL RYYGCGLVIP EHLENCWILD LGSGSGRDCY VLSQLVGEKG HVTGIDMTKG QVEVAEKYLD YHMEKYGFQA SNVTFIHGYI EKLGEAGIKN ESHDIVVSNC VINLVPDKQQ VLQEAYRVLK HGGELYFSDV YTSLELPEEI RTHKVLWGEC LGGALYWKEL AVLAQKIGFC PPRLVTANLI TIQNKELERV IGDCRFVSAT FRLFKHSKTG PTKRCQVIYN GGITGHEKEL MFDANFTFKE GEIVEVDEET AAILKNSRFA QDFLIRPIGE KLPTSGGCSA LELKDIITDP FKLAEESDSM KSRCVPDAAG GCCGTKKSC
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Molecular Weight
44 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
AS3MT (Arsenic (+3 oxidation state) Methyltransferase) is an enzyme critically involved in the metabolism of arsenic, a well-known environmental contaminant and toxic metalloid. The human AS3MT gene encodes a protein that catalyzes the methylation of inorganic arsenic, thereby facilitating its detoxification and excretion. This enzyme plays a pivotal role in the biotransformation process, converting arsenic into less toxic forms such as monomethylarsonic acid (MMA) and dimethylarsinic acid (DMA). Research on AS3MT is essential not only for understanding individual susceptibility to arsenic toxicity, which can lead to serious health issues including cancer, skin lesions, and cardiovascular diseases, but also for developing effective therapies and interventions for arsenic exposure. Genetic polymorphisms in the AS3MT gene have been linked to variations in arsenic metabolism among individuals, highlighting the importance of studying this enzyme in diverse populations. Moreover, AS3MT is also of interest in the context of environmental science, as it can influence the bioaccumulation of arsenic in food chains. Ongoing studies aim to elucidate the mechanistic pathways of AS3MT function, investigate its regulation, and explore potential therapeutic targets for minimizing arsenic-related health risks. Understanding the complexities of AS3MT's role in arsenic metabolism could provide critical insights into public health strategies aimed at mitigating the impact of arsenic exposure in affected communities. As arsenic contamination remains a significant global concern, research on AS3MT continues to be a focal point in both toxicology and environmental health disciplines, necessitating further investigation into its biochemical mechanisms and genetic variations.
