Analytical Data
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Gene name
COX3
- Application
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Alternative Names
COX3；COIII；COXIII；MTCO3；Cytochrome c oxidase subunit 3
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P23219
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Expression Region
24-599aa
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AA Sequence
ADPGAPT PVNPCCYYPC QHQGICVRFG LDRYQCDCTR TGYSGPNCTI PGLWTWLRNS LRPSPSFTHF LLTHGRWFWE FVNATFIREM LMRLVLTVRS NLIPSPPTYN SAHDYISWES FSNVSYYTRI LPSVPKDCPT PMGTKGKKQL PDAQLLARRF LLRRKFIPDP QGTNLMFAFF AQHFTHQFFK TSGKMGPGFT KALGHGVDLG HIYGDNLERQ YQLRLFKDGK LKYQVLDGEM YPPSVEEAPV LMHYPRGIPP QSQMAVGQEV FGLLPGLMLY ATLWLREHNR VCDLLKAEHP TWGDEQLFQT TRLILIGETI KIVIEEYVQQ LSGYFLQLKF DPELLFGVQF QYRNRIAMEF NHLYHWHPLM PDSFKVGSQE YSYEQFLFNT SMLVDYGVEA LVDAFSRQIA GRIGGGRNMD HHILHVAVDV IRESREMRLQ PFNEYRKRFG MKPYTSFQEL VGEKEMAAEL EELYGDIDAL EFYPGLLLEK CHPNSIFGES MIEIGAPFSL KGLLGNPICS PEYWKPSTFG GEVGFNIVKT ATLKKLVCLN TKTCPYVSFR VPDASQDDGP AVERPSTEL
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
COX3, or cytochrome c oxidase subunit 3, is a critical component of the mitochondrial respiratory chain, playing a vital role in cellular energy production through oxidative phosphorylation. It is encoded by mitochondrial DNA and is involved in the formation of the cytochrome c oxidase (CCO) complex, which catalyzes the reduction of oxygen to water, a process essential for ATP synthesis. Research on COX3 recombinant protein is significant due to its implications in understanding mitochondrial function and the pathogenesis of various mitochondrial diseases. Mutations in COX3 can lead to severe neurodegenerative disorders, highlighting the importance of studying this protein. Advances in recombinant protein technology allow for the generation of COX3 in vitro, facilitating detailed biochemical and structural analyses. This research not only contributes to a deeper understanding of mitochondrial bioenergetics but also aids in the development of potential therapies for diseases linked to mitochondrial dysfunction. By investigating the properties and dynamics of COX3, scientists aim to uncover novel insights into mitochondrial biology, which could lead to innovative strategies for diagnosing and treating mitochondrial-related conditions.
