Analytical Data
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基因名
WNT3
- Application
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别名
WNT3;INT4;Proto-oncogene Wnt-3
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P56703
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表达区间
1-355aa
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氨基酸序列
MEPHLLGLLLGLLLGGTRVLAGYPIWWSLALGQQYTSLGSQPLLCGSIPG LVPKQLRFCRNYIEIMPSVAEGVKLGIQECQHQFRGRRWNCTTIDDSLAI FGPVLDKATRESAFVHAIASAGVAFAVTRSCAEGTSTICGCDSHHKGPPG EGWKWGGCSEDADFGVLVSREFADARENRPDARSAMNKHNNEAGRTTILD HMHLKCKCHGLSGSCEVKTCWWAQPDFRAIGDFLKDKYDSASEMVVEKHR ESRGWVETLRAKYSLFKPPTERDLVYYENSPNFCEPNPETGSFGTRDRTC NVTSHGIDGCDLLCCGRGHNTRTEKRKEKCHCIFHWCCYVSCQECIRIYD VHTCK
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分子量
66 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
WNT3 is a key member of the Wnt signaling pathway, which plays a crucial role in various developmental processes, including cell proliferation, differentiation, and fate determination. The Wnt pathway is essential for embryonic development and has been implicated in the regulation of stem cell activity and the maintenance of pluripotency. Research into WNT3 has gained significance due to its involvement in various cancers and degenerative diseases, where aberrations in Wnt signaling can lead to uncontrolled cell growth and tumorigenesis. The production and characterization of recombinant WNT3 protein provide a valuable tool for studying its biological functions and the molecular mechanisms underlying its activity. This recombinant protein can be used in various assays to investigate its roles in cellular contexts, such as stem cell differentiation, tissue regeneration, and the modulation of immune responses. Additionally, understanding the structure and function of WNT3 can aid in the development of therapeutic strategies, including Wnt pathway inhibitors or activators, to treat diseases associated with dysregulated Wnt signaling. Overall, the study of WNT3 recombinant protein has the potential to contribute significantly to regenerative medicine, cancer therapy, and our overall understanding of developmental biology.












