Analytical Data
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Gene name
EAAT1
- Application
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Alternative Names
EAAT1；EAAT1；GLAST；GLAST1；Excitatory amino acid transporter 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P43003
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Expression Region
1-542aa
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AA Sequence
MTKSNGEEPKMGGRMERFQQGVRKRTLLAKKKVQNITKEDVKSYLFRNAFVLLTVTAVIVGTILGFTLRPYRMSYREVKYFSFPGELLMRMLQMLVLPLIISSLVTGMAALDSKASGKMGMRAVVYYMTTTIIAVVIGIIIVIIIHPGKGTKENMHREGKIVRVTAADAFLDLIRNMFPPNLVEACFKQFKTNYEKRSFKVPIQANETLVGAVINNVSEAMETLTRITEELVPVPGSVNGVNALGLVVFSMCFGFVIGNMKEQGQALREFFDSLNEAIMRLVAVIMWYAPVGILFLIAGKIVEMEDMGVIGGQLAMYTVTVIVGLLIHAVIVLPLLYFLVTRKNPWVFIGGLLQALITALGTSSSSATLPITFKCLEENNGVDKRVTRFVLPVGATINMDGTALYEALAAIFIAQVNNFELNFGQIITISITATAASIGAAGIPQAGLVTMVIVLTSVGLPTDDITLIIAVDWFLDRLRTTTNVLGDSLGAGIVEHLSRHELKNRDVEMGNSVIEENEMKKPYQLIAQDNETEKPIDSETKM
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Molecular Weight
59.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
EAAT1, or Excitatory Amino Acid Transporter 1, is a critical protein involved in the uptake of glutamate, the primary excitatory neurotransmitter in the central nervous system. Its role in regulating glutamate levels is essential for maintaining synaptic homeostasis, preventing excitotoxicity, and protecting against neurodegenerative diseases. Aberrant EAAT1 function has been linked to various neurological disorders, including epilepsy, Alzheimer's disease, and schizophrenia. Research on EAAT1 has gained momentum due to its potential as a therapeutic target; enhancing its function could mitigate glutamate-related neurotoxicity. Moreover, the understanding of EAAT1's structural and functional properties at the molecular level is crucial for drug discovery and development. Recombinant protein studies allow researchers to produce EAAT1 in a controlled environment, facilitating the investigation of its transport mechanisms, interaction with other proteins, and potential modulation by small molecules. The design of EAAT1-based therapies could lead to novel approaches in treating conditions characterized by glutamate dysregulation. Therefore, the exploration of EAAT1 recombinant protein holds significant promise for advancing our understanding of glutamate transporters and their implications in brain health and disease.
