Analytical Data
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Gene name
ACADM
- Application
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Alternative Names
ACADM；Medium-chain specific acyl-CoA dehydrogenase. mitochondrial
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P11310
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Expression Region
26-421aa
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AA Sequence
MGSSHHHHHHSSGLVPRGSHMKANRQREPGLGFSFEFTEQQKEFQATARK FAREEIIPVAAEYDKTGEYPVPLIRRAWELGLMNTHIPENCGGLGLGTFD ACLISEELAYGCTGVQTAIEGNSLGQMPIIIAGNDQQKKKYLGRMTEEPL MCAYCVTEPGAGSDVAGIKTKAEKKGDEYIINGQKMWITNGGKANWYFLL ARSDPDPKAPANKAFTGFIVEADTPGIQIGRKELNMGQRCSDTRGIVFED VKVPKENVLIGDGAGFKVAMGAFDKTRPVVAAGAVGLAQRALDEATKYAL ERKTFGKLLVEHQAISFMLAEMAMKVELARMSYQRAAWEVDSGRRNTYYA SIAKAFAGDIANQLATDAVQILGGNGFNTEYPVEKLMRDAKIYQIYEGTS QIQRLIVAREHIDKYKN
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Molecular Weight
46 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The study of recombinant ACADM (acyl-CoA dehydrogenase, medium-chain) protein is crucial due to its role in fatty acid metabolism, particularly in the catabolism of medium-chain fatty acids. ACADM is a key enzyme in the mitochondrial oxidation pathway, and mutations in the ACADM gene can lead to medium-chain acyl-CoA dehydrogenase deficiency (MCADD), a metabolic disorder associated with severe hypoglycemia and a risk of serious health complications, particularly in newborns and young children. The production of recombinant ACADM proteins allows researchers to better understand the enzyme's structure and function, investigate its interaction with various substrates, and explore the biochemical mechanisms underlying MCADD. Moreover, obtaining functional recombinant ACADM provides a platform for developing therapeutic strategies, including enzyme replacement therapies or small molecule drugs, aimed at treating the metabolic disruptions caused by ACADM deficiencies. As such, advances in the recombinant production and characterization of ACADM protein not only contribute to our fundamental understanding of metabolic pathways but also hold potential for translation into clinical applications that can improve patient outcomes.
