Analytical Data
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Gene name
ACADL
- Application
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Alternative Names
ACADL；Long-chain specific acyl-CoA dehydrogenase. mitochondrial
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P28330
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Expression Region
1-430aa
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AA Sequence
MAARLLRGSLRVLGGHRAPRQLPAARCSHSGGEERLETPSAKKLTDIGIR RIFSPEHDIFRKSVRKFFQEEVIPHHSEWEKAGEVSREVWEKAGKQGLLG VNIAEHLGGIGGDLYSAAIVWEEQAYSNCSGPGFSIHSGIVMSYITNHGS EEQIKHFIPQMTAGKCIGAIAMTEPGAGSDLQGIKTNAKKDGSDWILNGS KVFISNGSLSDVVIVVAVTNHEAPSPAHGISLFLVENGMKGFIKGRKLHK MGLKAQDTAELFFEDIRLPASALLGEENKGFYYIMKELPQERLLIADVAI SASEFMFEETRNYVKQRKAFGKTVAHLQTVQHKLAELKTHICVTRAFVDN CLQLHEAKRLDSATACMAKYWASELQNSVAYDCVQLHGGWGYMWEYPIAK AYVDARVQPIYGGTNEIMKELIAREIVFDK
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Molecular Weight
73 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ACADL (Acyl-CoA Dehydrogenase, Long-Chain) is a key enzyme involved in the metabolism of long-chain fatty acids, playing a crucial role in the mitochondrial fatty acid oxidation pathway. Deficiency of ACADL can lead to various metabolic disorders, including medium-chain acyl-CoA dehydrogenase deficiency, which is characterized by the inability to effectively break down long-chain fatty acids for energy production. Research into ACADL recombinant proteins has become increasingly important for understanding the structure-function relationship of this enzyme and its role in lipid metabolism. By producing recombinant ACADL, scientists can study its enzymatic activity, determine its substrate specificity, and investigate the effects of genetic mutations that may impair its function. Furthermore, recombinant ACADL can serve as a valuable tool for developing therapeutic strategies, such as enzyme replacement therapies for patients suffering from ACADL deficiency. This research not only enhances our understanding of fatty acid metabolism but also opens avenues for potential clinical applications, making the study of ACADL and its recombinant forms a significant focus in metabolic research.
