Analytical Data
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基因名
ACAA1
- Application
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别名
ACAA1；ACAA；PTHIO；3-ketoacyl-CoA thiolase. peroxisomal
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P09110
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表达区间
27-424aa
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氨基酸序列
MGSSHHHHHHSSGLVPRGSHMLSGAPQASAADVVVVHGRRTAICRAGRGG FKDTTPDELLSAVMTAVLKDVNLRPEQLGDICVGNVLQPGAGAIMARIAQ FLSDIPETVPLSTVNRQCSSGLQAVASIAGGIRNGSYDIGMACGVESMSL ADRGNPGNITSRLMEKEKARDCLIPMGITSENVAERFGISREKQDTFALA SQQKAARAQSKGCFQAEIVPVTTTVHDDKGTKRSITVTQDEGIRPSTTME GLAKLKPAFKKDGSTTAGNSSQVSDGAAAILLARRSKAEELGLPILGVLR SYAVVGVPPDIMGIGPAYAIPVALQKAGLTVSDVDIFEINEAFASQAAYC VEKLRLPPEKVNPLGGAVALGHPLGCTGARQVITLLNELKRRGKRAYGVV SMCIGTGMGAAAVFEYPGN
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分子量
44 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ACAA1, or Acetyl-CoA Acyltransferase 1, plays a crucial role in fatty acid metabolism, particularly in the final steps of mitochondrial fatty acid oxidation. This enzyme catalyzes the conversion of 3-ketoacyl-CoA to acetyl-CoA and acyl-CoA, which are vital intermediates in energy production. Research has shown that ACAA1 is essential for maintaining cellular energy homeostasis and regulating lipid metabolism. Mutations or dysregulation of ACAA1 have been implicated in various metabolic disorders, including mitochondrial diseases and obesity-related conditions. Understanding the structure and function of the ACAA1 recombinant protein provides insights into its enzymatic mechanisms and potential therapeutic applications. Recent studies focus on characterizing the recombinant form of ACAA1 through techniques such as crystallography and enzymatic assays, aiming to elucidate its catalytic properties and interaction with cofactors. These investigations not only enhance our understanding of fatty acid metabolism but also pave the way for developing targeted treatments for metabolic diseases associated with ACAA1 dysfunction. As a result, ACAA1 has gained significant interest in biochemical research, highlighting its potential as a biomarker and target for drug development.
