Analytical Data
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Gene name
LARGE
- Application
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Alternative Names
LARGE；KIAA0609；LARGE；Xylosyl- and glucuronyltransferase LARGE1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O95461
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Expression Region
1-756aa
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AA Sequence
MLGICRGRRKFLAASLSLLCIPAITWIYLFSGSFEDGKPVSLSPLESQAHSPRYTASSQRERESLEVRMREVEEENRALRRQLSLAQGRAPSHRRGNHSKTYSMEEGTGDSENLRAGIVAGNSSECGQQPVVEKCETIHVAIVCAGYNASRDVVTLVKSVLFHRRNPLHFHLIADSIAEQILATLFQTWMVPAVRVDFYNADELKSEVSWIPNKHYSGIYGLMKLVLTKTLPANLERVIVLDTDITFATDIAELWAVFHKFKGQQVLGLVENQSDWYLGNLWKNHRPWPALGRGYNTGVILLLLDKLRKMKWEQMWRLTAERELMGMLSTSLADQDIFNAVIKQNPFLVYQLPCFWNVQLSDHTRSEQCYRDVSDLKVIHWNSPKKLRVKNKHVEFFRNLYLTFLEYDGNLLRRELFGCPSEADVNSENLQKQLSELDEDDLCYEFRRERFTVHRTHLYFLHYEYEPAADSTDVTLVAQLSMDRLQMLEAICKHWEGPISLALYLSDAEAQQFLRYAQGSEVLMSRHNVGYHIVYKEGQFYPVNLLRNVAMKHISTPYMFLSDIDFLPMYGLYEYLRKSVIQLDLANTKKAMIVPAFETLRYRLSFPKSKAELLSMLDMGTLFTFRYHVWTKGHAPTNFAKWRTATTPYRVEWEADFEPYVVVRRDCPEYDRRFVGFGWNKVAHIMELDVQEYEFIVLPNAYMIHMPHAPSFDITKFRSNKQYRICLKTLKEEFQQDMSRRYGFAALKYLTAENNS
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Molecular Weight
88 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The study of LARGE recombinant protein is situated at the intersection of biotechnology and therapeutic development, primarily focusing on its role in glycosylation processes. LARGE is an essential gene that encodes an enzyme implicated in the elongation of heparan sulfate glycosaminoglycans. These glycosaminoglycans play critical roles in various biological processes, including cell signaling, proliferation, and tissue development. Mutations in the LARGE gene have been linked to congenital muscular dystrophy, specifically with a phenotype characterized by severe muscle weakness and mobility issues. Consequently, understanding the function and mechanisms of the LARGE protein is crucial for developing potential gene therapies and regenerative medicine strategies. Recent advancements in recombinant DNA technology have enabled the production of LARGE as a recombinant protein, facilitating in-depth studies of its structure and function. Research has shown that restoring NORMAL LARGE function can potentially ameliorate symptoms associated with muscular dystrophies in animal models. By exploring the biochemical pathways and interactions of LARGE protein, scientists aim to uncover new therapeutic targets and approaches for treating glycosylation disorders, ultimately contributing to personalized medicine frameworks that could improve patient outcomes. This line of research not only enhances our fundamental understanding of cellular processes but also holds promise for novel therapeutic interventions in genetic disorders linked to impaired glycosylation.
