Analytical Data
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基因名
MITF
- Application
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别名
MITF；BHLHE32；Microphthalmia-associated transcription factor
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O75030
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表达区间
1-520aa
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氨基酸序列
MLEMLEYNHYQVQTHLENPTKYHIQQAQRQQVKQYLSTTLANKHANQVLSLPCPNQPGDH VMPPVPGSSAPNSPMAMLTLNSNCEKEGFYKFEEQNRAESECPGMNTHSRASCMQMDDVI DDIISLESSYNEEILGLMDPALQMANTLPVSGNLIDLYGNQGLPPPGLTISNSCPANLPN IKRELTESEARALAKERQKKDNHNLIERRRRFNINDRIKELGTLIPKSNDPDMRWNKGTI LKASVDYIRKLQREQQRAKELENRQKKLEHANRHLLLRIQELEMQARAHGLSLIPSTGLC SPDLVNRIIKQEPVLENCSQDLLQHHADLTCTTTLDLTDGTITFNNNLGTGTEANQAYSV PTKMGSKLEDILMDDTLSPVGVTDPLLSSVSPGASKTSSRRSSMSMEETEHTC
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
MITF (Microphthalmia-associated transcription factor) is a critical transcription factor involved in various biological processes, including melanocyte development, pigmentation, and the maintenance of neural crest-derived cells. Its role in melanoma, a type of skin cancer, has garnered significant attention, as MITF is frequently found to be dysregulated in this disease. Research on MITF has revealed that it functions in a complex regulatory network, influencing cell differentiation and survival. The manipulation of MITF expression and activity offers potential therapeutic avenues for treating melanoma and other conditions linked to pigmentation anomalies. Recombination technologies used to generate MITF fusion proteins have provided essential insights into its functional domains and interactions with other cellular proteins. These studies are crucial for understanding the mechanisms underlying MITF-related diseases and for the development of targeted therapies. Furthermore, the exploration of MITF's role in cellular signaling pathways can shed light on its involvement in cancer progression and resistance to treatment. Overall, MITF and its recombinant protein studies represent a vital area of research that bridges basic science with potential clinical applications, offering hope for innovative strategies in cancer treatment and regenerative medicine.
