Analytical Data
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Gene name
cdtA
- Application
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Alternative Names
Cytolethal distending toxin subunit A(CDT A)
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Species
Escherichia coli
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Source
E. coli
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Tag
N- His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q46668
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Expression Region
22-258aa
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Molecular Weight
29.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The study of the cdtA recombinant protein is rooted in the exploration of its role in cellular processes and potential implications in disease pathology. CdtA, or cytolethal distending toxin A, is one of the components of the cytolethal distending toxin (CDT) family, produced by certain pathogens, including some strains of *Helicobacter pylori* and *Escherichia coli*. These toxins are known for their ability to interfere with the host cell cycle, leading to cellular distention and apoptosis. Research has revealed that cdtA plays a crucial role in the enzymatic activity of CDT, particularly in its interaction with host cell mechanisms. Through the expression of cdtA as a recombinant protein, scientists aim to better understand its structure, function, and the molecular mechanisms by which it exerts its toxic effects. This research has broader implications for developing therapeutic strategies against infections caused by CDT-producing bacteria, as well as gaining insights into the fundamental processes of cell cycle regulation and the host immune response. Further exploration of cdtA could lead to potential vaccine development or novel treatments for diseases linked to these pathogenic bacteria, highlighting its significance in both microbiology and medical research.
