Analytical Data
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Gene name
ptsH
- Application
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Alternative Names
Histidine-containing protein
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Species
Bacillus subtilis
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Source
E. coli
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Tag
N- His-SUMO
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P08877
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Expression Region
2-88aa
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Molecular Weight
25.1 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The research on PtsH recombinant protein is rooted in the growing interest in bacterial signaling networks and their implications for understanding cellular processes. PtsH, a component of the phosphotransferase system (PTS), plays a crucial role in carbohydrate metabolism and signal transduction in various bacteria. This protein is involved in the regulation of carbon catabolite repression, influencing how bacteria prioritize energy sources under different environmental conditions. Given its significance in microbial physiology, PtsH serves as a potential target for biotechnological applications, including the development of antimicrobial agents and metabolic engineering strategies. Additionally, the study of PtsH can provide insights into similar systems in other organisms, enhancing our understanding of evolutionary adaptations in metabolic pathways. The use of recombinant protein technology has facilitated the production and characterization of PtsH, allowing researchers to explore its function and interactions in vitro and in vivo. This research not only expands the fundamental knowledge of bacterial metabolism but also opens avenues for innovative applications in biotechnology and medicine.
