Analytical Data
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Gene name
Atp1a3
- Application
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Alternative Names
Sodium/potassium-transporting ATPase subunit alpha-3(Na(+)/K(+) ATPase alpha-3 subunit)(EC 7.2.2.13)(Na(+)/K(+) ATPase alpha(III) subunit)(Sodium pump subunit alpha-3)
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Species
Rat
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Source
E. coli
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Tag
N- His & C- Myc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P06687
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Expression Region
329-762aa
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Molecular Weight
54.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ATP1A3 is an essential gene that encodes the alpha-3 subunit of the sodium-potassium ATPase, an enzyme critical for maintaining ionic gradients across cellular membranes. This protein is predominantly expressed in the central nervous system and plays a significant role in neuronal excitability and signal transmission. Mutations in ATP1A3 have been linked to several neurological disorders, including Alternating Hemiplegia of Childhood (AHC) and other related syndromes. Research on recombinant ATP1A3 protein is crucial for understanding the physiological roles of this enzyme, elucidating the mechanisms by which mutations cause disease, and developing targeted therapies. Recombinant protein production allows for detailed biochemical characterizations, such as activity assays and structural analyses, which provide insights into the functional consequences of specific mutations. Additionally, studying ATP1A3 in a controlled laboratory environment enables the exploration of its interaction with other proteins and cellular components, further revealing its role in neuronal health and disease. Overall, research aimed at understanding ATP1A3 through recombinant protein methodologies is vital for advancing our knowledge of its function and potential therapeutic strategies for related neurological conditions.
