Analytical Data
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Gene name
cre
- Application
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Alternative Names
cre; Recombinase cre
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Species
Escherichia phage P1
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Source
E. coli
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Tag
N- His & C- Myc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P06956
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Expression Region
1-343aa
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Molecular Weight
46.0 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The research on Cre recombinase (Cre) proteins stems from their pivotal role in genetic engineering, particularly in the manipulation of DNA sequences within organisms. Discovered in the early 1980s, Cre, derived from the P1 bacteriophage, functions as a site-specific recombinase that recognizes loxP sites—short DNA sequences that enable the precise excision or insertion of genetic material. This capability has transformed numerous fields, including developmental biology, cancer research, and gene therapy, by allowing scientists to create conditional knockout models in mice and other organisms. The ability to control gene expression spatially and temporally has provided invaluable insights into gene function and regulation. Additionally, advancements in Cre technology have led to the development of various Cre-based systems, such as Cre-loxP, which allow for targeted gene activation or repression, enhancing the understanding of complex biological processes. The flexibility and efficiency of Cre recombinase have made it an indispensable tool in modern molecular biology, facilitating breakthroughs in our understanding of genetic elements and their roles in health and disease. As ongoing research continues to optimize Cre functionality and expand its applications, it remains a cornerstone of genetic manipulation techniques, paving the way for novel therapeutic approaches and innovations in synthetic biology.
