Analytical Data
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基因名
Gapdh1
- Application
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别名
Glyceraldehyde-3-phosphate dehydrogenase I (GAPDH I) (Gadph-1)
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种属
Drosophila melanogaster
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表达系统
E. coli
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标签
N- His-GST
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P07486
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表达区间
1-332aa
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分子量
66.9 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a well-characterized enzyme involved in glycolysis and plays a critical role in cellular metabolism. Beyond its metabolic functions, GAPDH is also implicated in various cellular processes, including apoptosis, oxidative stress response, and gene expression regulation, positioning it as a multifunctional protein. The study of recombinant GAPDH1 protein has gained attention due to its potential applications in research and biotechnology. Recombinant expression systems, such as E. coli or yeast, enable the production of high-quality GAPDH1 for structural and functional analyses. Research on GAPDH1 is particularly relevant in understanding its role in metabolic disorders, cancer progression, and neurodegenerative diseases. Furthermore, its utility as a reliable housekeeping gene for normalization in quantitative PCR amplifies its importance in molecular biology studies. The exploration of GAPDH1’s interactions with other cellular molecules and its post-translational modifications could unveil new therapeutic targets or biomarkers for disease. Given these diverse roles and applications, the investigation of recombinant GAPDH1 protein contributes to a better understanding of cellular metabolism and opens avenues for innovative biotechnological applications, such as drug development and metabolic engineering.












