Analytical Data
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Gene name
RAD52
- Application
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Alternative Names
RAD52; YML032C; DNA repair and recombination protein RAD52
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Species
Saccharomyces cerevisiae
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Source
E. coli
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Tag
N- His-SUMO
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P06778
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Expression Region
60-282aa
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Molecular Weight
41.1 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
RAD52 is a critical protein involved in homologous recombination, a fundamental mechanism for DNA repair and genomic stability. Research into RAD52 has gained momentum due to its role in repairing double-strand breaks, which are among the most detrimental types of DNA damage. Eukaryotic cells utilize RAD52 to facilitate the search for homologous sequences, thereby assisting in the accurate repair of DNA. Understanding RAD52 is particularly important in the context of cancer biology, as deficiencies or mutations in RAD52 and related proteins can lead to genomic instability, promoting tumorigenesis. Moreover, RAD52 has been implicated in various DNA damage response pathways, making it a potential target for therapeutic interventions, especially in cancers characterized by defects in other DNA repair mechanisms. Recent studies have also highlighted RAD52's interactions with other key proteins, such as RAD51 and BRCA proteins, underscoring its integrative role in the DNA repair network. Consequently, elucidating the molecular mechanisms of RAD52 not only enhances our understanding of cell biology but also opens new avenues for clinical applications in cancer treatment and genetic disorders.
