Analytical Data
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基因名
RAD52
- Application
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别名
RAD52; YML032C; DNA repair and recombination protein RAD52
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种属
Saccharomyces cerevisiae
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表达系统
E. coli
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标签
N- His-SUMO
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P06778
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表达区间
60-282aa
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分子量
41.1 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RAD52 is a critical protein involved in homologous recombination, a fundamental mechanism for DNA repair and genomic stability. Research into RAD52 has gained momentum due to its role in repairing double-strand breaks, which are among the most detrimental types of DNA damage. Eukaryotic cells utilize RAD52 to facilitate the search for homologous sequences, thereby assisting in the accurate repair of DNA. Understanding RAD52 is particularly important in the context of cancer biology, as deficiencies or mutations in RAD52 and related proteins can lead to genomic instability, promoting tumorigenesis. Moreover, RAD52 has been implicated in various DNA damage response pathways, making it a potential target for therapeutic interventions, especially in cancers characterized by defects in other DNA repair mechanisms. Recent studies have also highlighted RAD52's interactions with other key proteins, such as RAD51 and BRCA proteins, underscoring its integrative role in the DNA repair network. Consequently, elucidating the molecular mechanisms of RAD52 not only enhances our understanding of cell biology but also opens new avenues for clinical applications in cancer treatment and genetic disorders.
