Analytical Data
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Gene name
NSP12
- Application
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Species
Severe acute respiratory syndrome coronavirus 2
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Source
E. coli
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Tag
N- His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
YP_009725307.1
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Expression Region
1-932aa
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Molecular Weight
108.2 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
NSP12, the RNA-dependent RNA polymerase (RdRp), is a key enzyme responsible for the replication and transcription of coronaviruses, including SARS-CoV-2, which causes COVID-19. As a critical component of the viral replication machinery, NSP12 plays a vital role in the synthesis of viral RNA, making it a significant target for antiviral drug development. Understanding the structure and function of NSP12 is essential for elucidating the molecular mechanisms of coronavirus replication and for designing inhibitors that can effectively disrupt this process. Studies have shown that NSP12, in conjunction with its cofactors NSP7 and NSP8, forms a functional complex that enhances its polymerase activity. Furthermore, the fidelity of RNA synthesis by NSP12 has implications for the evolution of the virus, as errors in replication can lead to mutations. The recombinant production of NSP12 allows researchers to perform in-depth biochemical assays, structural analyses, and high-throughput screening of potential inhibitors, paving the way for novel therapeutic strategies to combat COVID-19. Moreover, as variants of the virus continue to emerge, ongoing research on NSP12 not only contributes to a better understanding of viral biology but also aids in the rapid development of vaccines and therapeutic agents, emphasizing the importance of NSP12 in the ongoing global health crisis.
