Analytical Data
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Gene name
plcH
- Application
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Alternative Names
Heat-labile hemolysin PLC-H Phosphatidylcholine cholinephosphohydrolase
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Species
Pseudomonas aeruginosa
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Source
E. coli
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Tag
N- His-SUMO & C- Myc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P06200
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Expression Region
51-436aa
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Molecular Weight
64.3 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The research on the recombinant protein PLCH (phospholipase C, delta isoform) is of significant interest due to its crucial role in various biological processes, including cell signaling, apoptosis, and proliferation. PLCH is an enzyme that hydrolyzes phosphoinositides, leading to the generation of inositol trisphosphate and diacylglycerol, important secondary messengers in cellular signaling pathways. Disruptions in PLCH function have been implicated in several diseases, including cancers and neurological disorders, making it a potential therapeutic target. Advances in recombinant DNA technology have facilitated the production of PLCH in heterologous systems, allowing researchers to study its structure, function, and interactions in greater detail. Understanding how PLCH operates at the molecular level can provide insights into its regulatory mechanisms and potential applications in drug design. Moreover, the characterization of PLCH's biochemical properties and its role in signal transduction networks enhances our comprehension of cellular physiology, making it an essential focus of biochemistry and molecular biology research. The growing interest in PLCH is also driven by the need for innovative strategies to manipulate its activity for therapeutic interventions, underlining the importance of recombinant protein studies in advancing biomedical science.
