Analytical Data
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Gene name
bipD
- Application
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Species
Burkholderia pseudomallei
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Source
E. coli
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Tag
N- His & C- Myc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q3JL26
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Expression Region
1-310aa
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Molecular Weight
41.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
BipD is a protein that plays a crucial role in the pathogenesis of several bacterial species, particularly in the context of host-pathogen interactions. It is part of the type III secretion system (T3SS), a sophisticated needle-like apparatus used by many Gram-negative bacteria to inject virulence factors directly into the host cells, facilitating infection and evasion of the immune response. The study of BipD focuses on its structural and functional characteristics, which are essential for understanding how it aids in the translocation of effector proteins. As an important component of the secretion apparatus, BipD is involved in the assembly and regulation of the T3SS, impacting the overall virulence of pathogens such as *Pseudomonas aeruginosa* and *Salmonella enterica*. Research on BipD has significant implications for developing new therapeutic strategies, as targeting the components of the T3SS could effectively inhibit bacterial infection. Additionally, characterizing the molecular interactions mediated by BipD may reveal insights into the mechanisms of bacterial adaptation and survival within host environments. Given the rising concern over antibiotic resistance, understanding the function of BipD not only enhances our basic knowledge of microbial pathogenesis but also paves the way for innovative approaches in combating bacterial diseases.
