Analytical Data
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Gene name
nrdA
- Application
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Alternative Names
Protein B1Ribonucleoside-diphosphate reductase 1 R1 subunitRibonucleotide reductase 1
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Species
Escherichia coli
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Source
E. coli
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Tag
N- His-SUMO
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P00452
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Expression Region
1-761aa
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Molecular Weight
101.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The study of nrdA recombinant protein is pivotal in understanding the biological functions and mechanisms of ribonucleotide reductase (RNR), an enzyme critical for DNA synthesis and repair. nrdA gene encodes the large subunit of the RNR enzyme, which catalyzes the reduction of ribonucleotides to deoxyribonucleotides, the building blocks of DNA. Given that this process is essential for cell proliferation and growth, deciphering the structure and function of nrdA is crucial for various applications, including cancer research and antibiotic development. The exploration of nrdA recombinant protein facilitates insights into the enzyme's catalytic mechanisms, regulatory pathways, and interactions with other cellular components. Additionally, due to its importance in the overall cellular metabolism, nrdA and its corresponding protein have emerged as attractive targets for novel therapeutic strategies. Thus, advancing our knowledge of nrdA and its recombinant protein can offer new avenues for drug design and contribute to the development of inhibitors that specifically target aberrant cellular proliferation in diseases such as cancer. Moreover, the ability to produce nrdA as a recombinant protein in a controlled laboratory setting enables detailed biochemical assays, structural studies, and high-throughput screening of potential inhibitors, which is fundamental for both academic research and biotechnological applications.
