Recombinant Vibrio parahaemolyticus serotype O3:K6 ompK Protein ，His & SUMO

The OmpK family of proteins, particularly OmpK35 and OmpK36, are outer membrane porins predominantly found in *Klebsiella pneumoniae,* a significant pathogen responsible for various infections, including pneumonia and bloodstream infections. These porins play a crucial role in the permeability of the bacterial outer membrane, regulating the passage of small molecules and contributing to antibiotic resistance. Understanding the structure and function of OmpK proteins is essential for developing novel therapeutic strategies, particularly in combating multidrug-resistant strains of *K. pneumoniae.* Recent studies have focused on the recombinant expression of OmpK proteins to facilitate detailed biochemical and biophysical analyses. This research is pivotal in elucidating the mechanisms of porin-mediated transport and resistance, and it may pave the way for engineered porins with enhanced functions or modified properties for biotechnological applications. Moreover, the increasing incidence of antibiotic resistance underscores the urgency in studying these porins to inform the design of new antibiotics or adjuvants that can restore the efficacy of existing drugs. Overall, the investigation of OmpK recombinant proteins stands at the intersection of microbiology, structural biology, and pharmacology, highlighting their potential implications in public health and infectious disease management.