Analytical Data
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Gene name
CYP74A
- Application
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Alternative Names
Cytochrome P450 74A Hydroperoxide dehydrase
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Species
Arabidopsis thaliana
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Source
E. coli
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Tag
N- His & C- Myc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q96242
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Expression Region
34-518aa
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Molecular Weight
61.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The CYP74A family of enzymes, particularly the CYP74A subgroup of cytochrome P450 monooxygenases, plays a significant role in the enzymatic conversion of fatty acids into functional metabolites involved in various physiological processes. These enzymes are crucial for the synthesis of volatile compounds, which are important for plant defenses, signaling, and interactions with the environment and pollinators. The study of recombinant CYP74A proteins offers insights into their catalytic mechanisms, substrate specificity, and potential applications in biotechnological processes. Research has demonstrated that these enzymes can metabolize a variety of fatty acids, producing compounds such as jasmonic acid and green leaf volatiles that are vital for plant stress responses and communication. Furthermore, understanding the structural and functional characteristics of CYP74A proteins can lead to advancements in synthetic biology, allowing for the engineering of metabolic pathways that enhance plant resilience or produce valuable bioactive compounds. Overall, investigating CYP74A recombinant proteins contributes to a deeper understanding of plant biochemistry and opens up avenues for improving agricultural practices and developing new bioproducts.
