Analytical Data
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Gene name
E189K
- Application
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Alternative Names
Verocytotoxin 2 subunit A Verotoxin 2 subunit A rRNA N-glycosidase 2
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Species
Enterobacteria phage 933W
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Source
E. coli
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Tag
N- His & C- Myc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P09385
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Expression Region
23-319aa(E189K)
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Molecular Weight
40.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The research on the E189K recombinant protein has gained momentum due to its potential implications in understanding various biological processes and diseases. E189K is a mutation found in certain proteins that has been linked to alterations in their structure and function, particularly in relation to their role in disease mechanisms, such as cancer and neurodegenerative disorders. The importance of studying this specific variant lies in its ability to provide insights into protein stability, interaction with other biomolecules, and the pathways influenced by these changes. By generating and characterizing the E189K recombinant protein, researchers aim to elucidate the molecular consequences of this mutation, investigate its impact on cellular functions, and explore therapeutic strategies to counteract its effects. This research not only contributes to the fundamental understanding of protein dynamics but also paves the way for the development of targeted treatments that could mitigate the adverse effects associated with E189K mutations.
