Analytical Data
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Gene name
LSS
- Application
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Alternative Names
2,3-epoxysqualene--lanosterol cyclase Oxidosqualene--lanosterol cyclase
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Species
Human
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Source
E. coli
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Tag
N- His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P48449
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Expression Region
2-732aa
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Molecular Weight
89.2 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
LSS, or Lanosterol Synthase, is an essential enzyme involved in the biosynthesis of sterols, playing a critical role in the cholesterol synthesis pathway. This enzyme catalyzes the conversion of 2,3-oxidosqualene to lanosterol, serving as a precursor for multiple steroid compounds. Research into LSS has gained momentum due to its implications in various biological and pathological processes, including cellular signaling, membrane dynamics, and the development of certain diseases. Mutations or dysregulation of LSS have been linked to disorders such as congenital bile acid synthesis defects and various forms of cancer, making it a potential therapeutic target. Additionally, understanding the structural and functional aspects of LSS can provide insights into the evolutionary adaptations of steroid metabolism across different organisms. As such, the study of LSS and its recombinant forms has become an important area of investigation, focusing on elucidating its enzymatic mechanisms, regulation, and interaction with inhibitors. The production of recombinant LSS provides a means to explore its characteristics in vitro, paving the way for the development of novel compounds that could influence cholesterol metabolism and related diseases. This growing body of research underscores the significance of LSS not only as a fundamental enzyme in steroid biosynthesis but also as a promising target for therapeutic intervention in metabolic disorders.
