Analytical Data
-
Gene name
SIZ1
- Application
-
Alternative Names
E3 SUMO-protein transferase SIZ1
-
Species
Arabidopsis thaliana
-
Source
E. coli
-
Tag
N- His
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
Q680Q4
-
Expression Region
1-171aa
-
Molecular Weight
23.2 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
SIZ1 (Suppressor of the Zeste 12) is a unique protein involved in the regulation of several cellular processes, particularly in plants. It is an SUMO (Small Ubiquitin-like Modifier) E3 ligase, which plays a crucial role in post-translational modifications by facilitating the attachment of SUMO molecules to target proteins, thereby influencing their stability, localization, and activity. Research on SIZ1 has gained traction due to its implications in various physiological and stress-related responses, including the regulation of flowering time, responses to environmental stresses, and defense mechanisms against pathogens. Studies have shown that mutations in SIZ1 can lead to altered growth patterns and stress responses, highlighting its importance in plant development and adaptation. The exploration of SIZ1’s functions also provides insights into the broader significance of SUMOylation in eukaryotic cells. Understanding SIZ1 and its associated pathways not only advances basic plant biology but also offers potential applications in agricultural biotechnology, such as developing crops with improved resilience to climate change and biotic stresses. Researchers continue to investigate the molecular mechanisms underlying SIZ1’s activities, aiming to elucidate its interactions with various signaling pathways and its role in plant metabolism, which could ultimately lead to more sustainable agricultural practices.
