Cat: IPD-X40109

Recombinant Leuconostoc mesenteroides Dextransucrase Protein ,His & KSI

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Analytical Data

  • Gene name

    Dextransucrase

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    Glucansucrase (Sucrose 6-glucosyltransferase)

  • Species

    Leuconostoc mesenteroides

  • Source

    E. coli

  • Tag

    N- His-KSI

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    P86897

  • Expression Region

    1-9aa

  • Molecular Weight

    16.3 kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

Quality inspection process

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Protein Description

Dextransucrase is an enzyme produced by various strains of bacteria, particularly from the genus Leuconostoc, known for its ability to catalyze the conversion of sucrose into dextran, a polysaccharide composed of glucose units. This enzyme plays a significant role in the food industry, especially in the production of gummy candies and in enhancing the texture of food products. Its unique properties have made it a subject of extensive research, particularly in the context of biotechnological applications, where understanding its structure and function can lead to advancements in food preservation and fermentation processes. Additionally, dextrans produced by dextransucrase have valuable medical applications, including their use as blood volume expanders and in drug delivery systems. With the potential to generate recombinant forms of this enzyme, researchers are exploring genetic engineering techniques to enhance its activity and stability, optimize its production, and tailor its properties for specific industrial needs. The study of dextransucrase and its recombinant proteins not only provides insights into enzyme kinetics and mechanisms but also opens up new pathways for innovation in enzyme technology and applied microbiology.

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