Analytical Data
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Gene name
UBE2D3
- Application
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Alternative Names
Ubiquitin carrier protein D3Ubiquitin-conjugating enzyme E2(17)KB 3Ubiquitin-conjugating enzyme E2-17KDA 3Ubiquitin-protein ligase D3
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Species
Human
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Source
E. coli
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Tag
N- His-SUMO
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P61077
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Expression Region
1-147aa
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Molecular Weight
32.7 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
UBE2D3, a member of the E2 ubiquitin-conjugating enzyme family, plays a crucial role in the ubiquitin-proteasome system, which regulates protein degradation and various cellular processes such as cell cycle progression, apoptosis, and DNA repair. Research has highlighted UBE2D3's involvement in the ubiquitination of key substrates, influencing signal transduction pathways and contributing to the maintenance of cellular homeostasis. Given its significant role in regulating protein stability and its implication in various diseases, including cancer, neurological disorders, and viral infections, the recombinant expression and characterization of UBE2D3 have emerged as a vital area of investigation. By generating recombinant UBE2D3, researchers aim to better understand its enzymatic mechanisms, substrate specificity, and regulatory interactions. Furthermore, understanding the structural and functional aspects of UBE2D3 may pave the way for the development of novel therapeutic strategies targeting the ubiquitin-proteasome pathway, potentially offering new insights for disease treatment and management. The ongoing research into UBE2D3 underscores its potential as a biomarker and therapeutic target, as well as its importance in elucidating the complexities of cellular regulation through ubiquitination.
