Analytical Data
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Gene name
TK2
- Application
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Alternative Names
Mt-TK
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Species
Human
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Source
E. coli
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Tag
N- His & C- Myc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O00142
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Expression Region
34-265aa
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Molecular Weight
32.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The study of TK2 (thymidine kinase 2) recombinant protein has gained significant attention due to its crucial role in the mitochondrial salvage pathway of nucleotide metabolism. TK2 is an enzyme responsible for phosphorylating thymidine and deoxycytidine, thereby converting them into their phosphorylated forms, which are essential for DNA synthesis and repair within mitochondria. Genetic mutations in the TK2 gene can lead to severe mitochondrial diseases, commonly known as mitochondrial DNA depletion syndromes, which result in neurological deficits and muscular dystrophies. Understanding the structure and function of TK2 is essential for developing therapeutic strategies and gene therapies for these conditions. Recombinant TK2 protein offers a valuable tool for biochemical studies aimed at elucidating its enzymatic mechanisms, interactions, and potential as a therapeutic target. Additionally, investigating the recombinant form enables researchers to explore its role in cellular metabolism and to assess the effects of specific genetic mutations on its activity. Overall, research into TK2 recombinant protein not only enhances our understanding of mitochondrial biology but also contributes to the broader field of gene therapy, with the potential to address critical health issues related to mitochondrial dysfunction.
