Analytical Data
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基因名
PRIM1
- Application
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别名
DNA primase 49KDA subunit Short name: p49
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种属
Human
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表达系统
E. coli
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标签
N- His-SUMO
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P49642
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表达区间
1-420aa
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分子量
65.9 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PRIM1, or Primase 1, is a pivotal enzyme involved in DNA replication, specifically in the synthesis of RNA primers essential for the initiation of DNA strand synthesis. Research on PRIM1 has intensified due to its critical role in maintaining genomic stability, which is vital for cellular proliferation and organismal development. Mutations or dysregulation of PRIM1 can lead to replication stress, contributing to various diseases, including cancer. Understanding the structure and function of PRIM1 and its interactions with other replication factors can provide insights into the mechanisms of DNA replication and repair. Additionally, PRIM1 serves as a potential target for therapeutic interventions, as manipulating its activity may enhance the effectiveness of chemotherapeutic agents. Notably, investigations into the recombinant expression of PRIM1 facilitate the production of large quantities of the protein, enabling detailed biochemical and biophysical studies. These studies aim to elucidate the enzyme's mechanism of action, its regulation, and its role in the broader context of DNA metabolism. Through these efforts, researchers hope to better understand the molecular underpinnings of diseases associated with DNA replication failures and to develop innovative strategies for treatment and prevention.
