Analytical Data
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基因名
POU5F1
- Application
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别名
Octamer-binding protein 3
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种属
Human
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表达系统
E. coli
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标签
N- His-B2M
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q01860
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表达区间
1-360aa
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分子量
52.6 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
POU5F1, also known as Oct4, is a crucial transcription factor that plays a significant role in maintaining pluripotency and self-renewal in embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). It belongs to the POU (Pit-Oct-Unc) family of proteins and is characterized by its ability to bind to specific DNA sequences, thereby regulating genes essential for stem cell characteristics. Research surrounding POU5F1 has garnered considerable attention due to its implications in developmental biology, regenerative medicine, and cancer research. The understanding of POU5F1's function has paved the way for advancements in stem cell therapies, as manipulating its expression can influence cell fate decisions. Additionally, aberrant expression of POU5F1 has been linked to various cancers, where it may contribute to the maintenance of cancer stem cell populations. The production and characterization of recombinant POU5F1 protein are vital for elucidating its biochemical properties and interactions, facilitating in-depth studies on its role in stem cell biology and tumorigenesis. Recombinant POU5F1 protein can serve as a valuable tool in high-throughput screening and therapeutic development, offering insights into the molecular mechanisms underlying stem cell maintenance and differentiation. As such, continued research on POU5F1 not only enhances our understanding of stem cell regulation but also opens potential avenues for innovative treatments across diverse fields, including developmental disorders and cancer intervention.
