Analytical Data
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基因名
Frizzled-7
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简介
Frizzled-7 (FZD7) is a Wnt receptor that primarily activates the β-catenin classical pathway, involving Disheveled protein, GSK-3 kinase inhibition, nuclear β-catenin accumulation, and Wnt target gene activation. Some family members exhibit a second PKC and calcium flux signaling pathway, but the integration is unclear. Frizzled-7/FZD7 Protein, Human (HEK293, His) is the recombinant human-derived Frizzled-7/FZD7 protein, expressed by HEK293 , with C-His labeled tag.
- Application
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别名
FZD7; Frizzled-7; FzE3; Fz-7; hFz7
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种属
Human
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表达系统
HEK293
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标签
C-6*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O75084
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表达区间
Q33-L185
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蛋白长度
Partial
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分子量
23-33 kDa.
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Frizzled-7 (Fz7) is a member of the Frizzled family of proteins, which are integral to the Wnt signaling pathway, a crucial mechanism in various developmental processes and tissue homeostasis. In recent years, research has focused on Fz7 due to its significant role in the regulation of cellular functions such as cell proliferation, differentiation, and polarity. Dysregulation of Wnt signaling, often mediated by Frizzled receptors like Fz7, has been implicated in numerous diseases, including cancers and developmental disorders. Therefore, understanding the structure and function of Fz7 is paramount for elucidating the molecular mechanisms underpinning these pathologies. The study of Fz7 recombinant proteins has gained traction as it allows researchers to investigate the receptor's binding affinities, signaling capabilities, and interactions with Wnt ligands and other intracellular partners in a controlled environment. Additionally, recombinant Fz7 proteins can serve as valuable tools for the development of therapeutic strategies aimed at modulating Wnt signaling. By employing techniques such as protein expression in heterologous systems and subsequent purification, researchers aim to characterize the biochemical properties of Fz7 further. This knowledge can pave the way for potential interventions in conditions associated with aberrant Wnt signaling, highlighting the importance of Frizzled-7 in both basic biology and clinical applications.
