Analytical Data
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Gene name
GADD45A/DDDIT-1
- Application
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Alternative Names
DDIT1; GADD45; DNA damage-inducible transcript 1 protein
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
P24522
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Expression Region
Met1~Arg165
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Molecular Weight
24kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
GADD45A (Growth Arrest and DNA Damage-Inducible Protein 45 Alpha) is a key regulator of cellular responses to environmental stressors, including DNA damage, and plays a crucial role in maintaining genomic integrity. The protein is involved in various cellular processes such as cell cycle regulation, apoptosis, and differentiation. DDDIT-1, also known as Gadd45a-interacting protein, has been identified as a potential partner that enhances the functional repertoire of GADD45A. Research into the recombinant expression of GADD45A and its interaction with DDDIT-1 is driven by the need to understand their roles in cancer biology and stress response mechanisms. By studying the recombinant forms of these proteins, researchers aim to elucidate their molecular mechanisms and functional pathways through biochemical assays and structural analyses. This knowledge could form the basis for developing novel therapeutic strategies targeting GADD45A/DDDIT-1 interactions to modulate cellular responses in diseases characterized by aberrant DNA repair and cell cycle dysregulation, particularly in cancer. Understanding the intricate interplay between these proteins may not only shed light on fundamental biological processes but also pave the way for potential interventions in cancer treatment and prevention. Overall, the study of GADD45A and DDDIT-1, especially in their recombinant forms, holds significant promise for advancing our understanding of stress response and maintaining cellular homeostasis in the face of genotoxic stress.
