Analytical Data
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Gene name
CLOCK
- Application
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Alternative Names
KAT13D; bHLHe8; Class E basic helix-loop-helix protein 8
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O15516
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Expression Region
Ala34~Ala379
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Molecular Weight
44kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The CLOCK protein is a central component of the circadian clock, a complex timekeeping system that regulates various physiological processes in organisms, including sleep-wake cycles, hormone release, and metabolism. As a part of the basic helix-loop-helix (bHLH) family of transcription factors, CLOCK interacts with other proteins, such as BMAL1, to form a heterodimer that activates the expression of clock-controlled genes. Research on CLOCK has gained significant traction due to its critical role in maintaining circadian rhythms and its implications in various health conditions, including sleep disorders, metabolic syndromes, and mood disorders. Understanding the structure and function of CLOCK protein, along with its regulatory mechanisms, is essential for developing therapeutic strategies to address these conditions. Recent studies have focused on CLOCK’s post-translational modifications, such as phosphorylation and acetylation, which can influence its stability, localization, and transcriptional activity. Moreover, the interplay between CLOCK and environmental factors, such as light and temperature, underscores the adaptability of circadian rhythms and raises intriguing questions about the evolutionary significance of these timekeeping mechanisms. As circadian biology continues to evolve as a field, CLOCK protein research not only sheds light on fundamental biological processes but also opens avenues for innovative treatments and interventions in chronobiology-related health issues.
