Analytical Data
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Gene name
NKX2-5
- Application
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Alternative Names
CSX; NKX2E; CSX1; NKX2.5; NKX4-1; Tinman Paralog; Cardiac-Specific Homeo Box; NK2 Transcription Factor Related Protein, Locus 5
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P52952
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Expression Region
Gln24~Arg137
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Molecular Weight
16kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NKX2-5, a member of the NK-2 family of transcription factors, plays a crucial role in cardiac development and function. It is predominantly expressed in the heart, specifically during embryogenesis, where it regulates cardiac progenitor cell differentiation and the formation of the heart's structural components. Mutations or dysregulation of NKX2-5 are associated with various congenital heart defects and arrhythmias, making it a significant focus of research in cardiology and developmental biology. The study of NKX2-5 recombinant proteins has gained traction as a means to elucidate its functional mechanisms and interactions with other molecules involved in heart development. By creating recombinant NKX2-5 proteins, researchers can investigate the effects of specific domain mutations, assess protein-protein interactions, and explore their impacts on gene expression in vitro. Furthermore, understanding the structural biology of NKX2-5 offers insights into the molecular basis of its role in heart formation and its potential as a therapeutic target. Such research has implications not only for congenital heart disease but also for regenerative medicine and tissue engineering, as insights gained could inform approaches to repair or regenerate damaged heart tissue. Overall, the exploration of NKX2-5 recombinant proteins is pivotal for advancing our knowledge of cardiac biology and developing novel therapeutic strategies for heart-related disorders.
