Analytical Data
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Gene name
CENPB
- Application
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Alternative Names
(Centromere protein B)(CENP-B)
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Species
Human
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Source
E. coli
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Tag
N- His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P07199
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Expression Region
2-599aa
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Molecular Weight
71.0 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
CENPB, or Centromere Protein B, is a critical component of the kinetochore, essential for proper chromosome segregation during cell division. As a DNA-binding protein, CENPB plays a vital role in securing centromeric DNA and facilitating the interaction with microtubules, thus ensuring accurate chromosome alignment and segregation in mitosis and meiosis. Research into CENPB has gained prominence due to its implications in various human diseases, including cancer. Aberrant expression or mutation of CENPB can lead to aneuploidy and genomic instability, common features of malignant tumors. The development of recombinant CENPB protein allows for in-depth studies of its structure, function, and interactions with other proteins at the kinetochore. Purified CENPB can be utilized in biochemical assays to elucidate its role in centromere dynamics and to investigate potential therapeutic targets in cancer treatment. Furthermore, understanding the mechanisms by which CENPB contributes to chromosomal stability can provide insights into fundamental biological processes and pave the way for novel approaches in managing diseases characterized by genomic instability. Thus, the study of CENPB not only enhances our understanding of cell biology but also represents a promising avenue for advancing cancer research and therapeutics.
