Analytical Data
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Gene name
GLIS1
- Application
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Alternative Names
GLI-similar 1
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8NBF1
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Expression Region
Met446~Thr620
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Molecular Weight
22kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
GLIS1, a member of the GLIS (Gli-similar) family of transcription factors, has garnered significant interest in recent years due to its crucial role in various biological processes, including embryonic development and stem cell regulation. This protein is particularly important in the context of organogenesis and cellular differentiation, as it modulates gene expression by interacting with specific DNA sequences and recruiting various co-factors. Research has shown that GLIS1 is involved in processes such as nephrogenesis, which is the formation of kidneys, and it has been linked to a number of developmental disorders when dysregulated. The ability to produce recombinant GLIS1 protein has opened new avenues for understanding its structure-function relationships and biological activities. By creating GLIS1 in a laboratory setting, researchers can study its properties in detail, including its binding affinity to DNA and interactions with other proteins. This research is crucial for elucidating the pathways mediated by GLIS1 and may provide insights into potential therapeutic strategies for conditions associated with its dysfunction. Moreover, the study of GLIS1 in the context of regenerative medicine, particularly in kidney repair and regeneration, highlights its potential clinical significance. Overall, the investigation of GLIS1 recombinant protein is a vital step toward unraveling the complexities of developmental biology and its implications for human health.
