Analytical Data
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Gene name
GM2A
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简介
GM2A Protein is a small glycolipid transport protein which acts as a substrate specific co-factor for the lysosomal enzyme beta-hexosaminidase A. Beta-hexosaminidase A, together with GM2A, catalyzes the degradation of the ganglioside GM2, and other molecules containing terminal N-acetyl hexosamines. And it is a lipid transfer protein that stimulates the enzymatic processing of gangliosides, and also T-cell activation through lipid presentation. GM2A Protein, Human (sf9, His) is the recombinant human-derived GM2A protein, expressed by Sf9 insect cells , with C-His labeled tag.
- Application
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Alternative Names
Ganglioside GM2 activator; GM2-AP; Sphingolipid activator protein 3; SAP-3
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Species
Human
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Source
Baculovirus
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Tag
C-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
AAA35907.1
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Expression Region
H24-I193
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Protein Length
Full Length of Mature Protein
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Molecular Weight
25 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
GM2A, or GM2 ganglioside activator protein, is a vital component in the metabolism of gangliosides, particularly in the degradation of GM2 gangliosides. Mutations in the GM2A gene can lead to GM2 gangliosidosis, a serious neurodegenerative disorder characterized by the accumulation of GM2 gangliosides in the brain, ultimately resulting in severe neurological symptoms. The study of GM2A recombinantly expressed proteins has gained significant attention in recent years due to its implications in understanding the pathophysiology of GM2 gangliosidosis and potential therapeutic applications. Research has focused on elucidating the structure and function of GM2A, its interactions with other proteins involved in lipid metabolism, and its role in cellular pathways associated with ganglioside degradation. By using recombinant DNA technology to express GM2A protein, scientists can generate sufficient quantities for detailed biochemical studies and therapeutic development. Additionally, this research could pave the way for enzyme replacement therapies and innovative treatment strategies for patients suffering from GM2 gangliosidosis, as well as enhance our understanding of lipid metabolism disorders more broadly. The exploration of GM2A's functional mechanisms holds promise for advancing both basic and translational research in the field of neurobiology and metabolic diseases.
