Analytical Data
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Gene name
Semaphorin-5A/SEMA5A
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简介
Semaphorin-5A/SEMA5A proteins act as bifunctional axon guidance signals, exerting attraction via heparan sulfate proteoglycans and inhibitory effects via chondroitin sulfate proteoglycans. As a ligand for the PLXNB3 receptor, SEMA5A disrupts glioma cell migration and invasion and induces cell collapse. Semaphorin-5A/SEMA5A Protein, Human (HEK293, Fc) is the recombinant human-derived Semaphorin-5A/SEMA5A protein, expressed by HEK293 , with C-hFc labeled tag.
- Application
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Alternative Names
Semaphorin-5A; Semaphorin-F; Sema F; SEMA5A; SEMAF
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Species
Human
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Source
HEK293
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Tag
C-hFc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q13591
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Expression Region
M1-T765
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Protein Length
Partial
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Molecular Weight
125-135 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Semaphorin-5A (SEMA5A) is a member of the semaphorin family, which plays crucial roles in neuronal development and guidance. Initially identified for its function in cellular signaling during neural development, SEMA5A is known to interact with specific receptors, influencing processes such as axon guidance, synapse formation, and cell migration. Recent studies have expanded its importance beyond the nervous system, suggesting its involvement in various physiological and pathological processes, including immune response, cardiovascular development, and cancer progression. The investigation of recombinant SEMA5A proteins has gained traction as researchers aim to characterize its structural properties and functional roles in both normal and disease states. The generation of these recombinant proteins allows for in-depth studies on SEMA5A's mechanisms of action, receptor interactions, and potential therapeutic applications. Moreover, understanding the signaling pathways regulated by SEMA5A can facilitate the development of novel treatments targeting neurodevelopmental disorders, immune diseases, and tumor progression. Overall, the study of recombinant SEMA5A proteins holds promise for elucidating its diverse biological functions and therapeutic potential.
